Binding of some antibacterial fluoroquinolones with ds-DNA, β-cyclodextrin and hemoglobin: spectroscopic and voltammetric studies

Ibrahim A. Al-hagri
1Department of Chemistry, College of Science, Qassim University, Buraidh, Saudi Arabia.
Yassien M. Temerk
Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Sadeq M. Al-Hazmy
Department of Chemistry, College of Science, Qassim University, Buraidh, Saudi Arabia.
Mustafa M. Kamal
Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Mohamed S. Ibrahim
Chemistry Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Nabil A. Al-hemiary
Department of Chemistry , Faculty of Science , Ibb University, P. O. Box 70270, Ibb, Yemen
Ahmed N. Al-Hakemi
Department of Chemistry, College of Science, Qassim University, Buraidh, Saudi Arabia.
Hassan Elobeid Ahmed
Department of Chemistry, College of Science, Qassim University, Buraidh, Saudi Arabia.
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Abstract

The interaction of some antibacterial fluoroquinolones (FQ), Difloxacin (DIX), Danofloxacin (DANO) and Orbifloxacin (ORBX) drugs, with ds-DNA, β-Cyclodextrin (β-CD) and hemoglobin was investigated by UV-Vis spectroscopy, cyclic voltammetric and differential pulse voltammetry. The absorption spectral and electrochemical data show that DIX or DANO or ORBX, acting an intercalator, is inserted into the base-stacking domain of the ds-DNA. Structural effects of investigated fluoroquinolones on their binding to ds-DNA were discussed. The interaction of inclusion complex of DIX- β-CD, DANO- β-CD and ORBX- β-CD with ds-DNA is reported. The nature of the process of hemoglobin and DIX, DANO and ORBX was clarified. The binding affinity and thermodynamic parameters of DIX, DANO and ORBX with the host molecule were computed. The calibration graph for the determination of ds-DNA, β-CD and hemoglobin was obtained by the decrease in the differential pulse peak current of the investigated fluoroquinolones in the presence of the host molecule.

Table of Contents
Al-hagri, I. A., Temerk, Y. M. ., Al-Hazmy, S. M., Kamal, M. M. ., Ibrahim, M. S., Al-hemiary, N. A., Al-Hakemi, A. N., & Ahmed, H. E. (2022). Binding of some antibacterial fluoroquinolones with ds-DNA, β-cyclodextrin and hemoglobin: spectroscopic and voltammetric studies. Journal of Qassim University for Science, 13(1), 24–41. Retrieved from https://jnsm.qu.edu.sa/index.php/jnm/article/view/2291
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